pGEX Plasmids

pGEX plasmids are a family of expression vectors designed for high-yield production of glutathione-S-transferase (GST)-fusion proteins in Escherichia coli. The fusion of GST to a target protein provides a convenient way to enhance solubility, simplify purification through glutathione affinity chromatography, and enable functional assays. These plasmids are widely used in molecular biology, biochemistry, and structural biology for studying protein function, interactions, and structure.

Key Features of pGEX Plasmids
  • Multiple Cloning Site (MCS):
    • Located downstream of the GST sequence, enabling the insertion of the gene of interest to create a GST-fusion protein.
  • Thrombin or Factor Xa Cleavage Sites:
    • Included between the GST tag and the MCS to allow removal of the GST tag after purification if desired.
  • Ampicillin Resistance (ampR):
    • Provides selection in E. coli using ampicillin.
  • Replication Origin (Ori):
    • Derived from pBR322, allowing moderate plasmid copy number.
  • Size:
    • Approximately 4.9–5.0 kb, depending on the specific variant.
Applications of pGEX Plasmids
  • Protein Purification:
    • Simplifies purification of recombinant proteins using glutathione-Sepharose affinity chromatography.
  • Protein Solubility Enhancement:
    • GST tag improves the solubility of difficult-to-express proteins, reducing aggregation in E. coli.
  • Protein-Protein Interaction Studies:
    • GST-fusion proteins are used in pull-down assays to identify binding partners and study interactions.
  • Enzymatic Activity Studies:
    • Retains enzymatic activity of GST, enabling fusion proteins to bind and release from glutathione-based resins under mild conditions.
  • Antibody Production:
    • GST-fusion proteins are used as antigens for generating specific antibodies.

pGEX plasmids are highly effective tools for expressing, purifying, and studying recombinant proteins in E. coli. Their GST-fusion system simplifies purification, enhances solubility, and supports versatile downstream applications, such as functional and structural analyses. These plasmids are a mainstay in molecular biology and biochemistry research, offering reliable and scalable protein expression solutions.

AffiPLASMID® pGEX-TagRFP-6XHis
CAT# AFG-NVF-100266
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-Nanometer Antibody-ftnA
CAT# AFG-NVF-100265
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-ftnA-6xHis
CAT# AFG-NVF-100264
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-ftnA
CAT# AFG-NVF-100263
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-6P-PTEN
CAT# AFG-NVF-100262
Size: 2 μg
846.00 846.0 USD
AffiPLASMID® pGEX-6P-1-USP21 Plasmid
CAT# AFG-NVF-100261
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-6P-1-RABEP1
CAT# AFG-NVF-100260
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-6P-1-Kan
CAT# AFG-NVF-100259
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-6P-1-GST-NFYA
CAT# AFG-NVF-100258
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX6P1-GST-CEBPB
CAT# AFG-NVF-100257
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX6P1-GFP-Nanobody
CAT# AFG-NVF-100256
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-6P-1-FTH1
CAT# AFG-NVF-100255
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-6P-1-FAM50A
CAT# AFG-NVF-100254
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-6P-1-FAM129B-RQ-AA
CAT# AFG-NVF-100253
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-6P1-BACH1-S196A Plasmid
CAT# AFG-NVF-100252
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-6P1-BACH1 Plasmid
CAT# AFG-NVF-100251
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX6p1-AICL Plasmid
CAT# AFG-NVF-100250
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-6P-1-6
CAT# AFG-NVF-100249
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-5X-2 plasmid
CAT# AFG-NVF-100248
Size: 2 μg
368.40 368.40000000000003 USD
AffiPLASMID® pGEX-5X-1
CAT# AFG-NVF-100247
Size: 2 μg
368.40 368.40000000000003 USD
AffiPLASMID® pGEX4T-Tac-VAMP2-Linker-GST
CAT# AFG-NVF-100246
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX4T-Tac-VAMP1-Linker-GST
CAT# AFG-NVF-100245
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX4T-Tac-STX1A-Linker-GST
CAT# AFG-NVF-100244
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX4T-Tac-SNAP25-Linker-GST
CAT# AFG-NVF-100243
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX4T-Tac-MCS-Linker-GST
CAT# AFG-NVF-100242
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX4T-Tac-MCS
CAT# AFG-NVF-100241
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-STK19
CAT# AFG-NVF-100240
Size: 2 μg
846.00 846.0 USD
AffiPLASMID® pGEX-4T-PLK1
CAT# AFG-NVF-100238
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-PKLR
CAT# AFG-NVF-100237
Size: 2 μg
726.00 726.0 USD
AffiPLASMID® pGEX-4T-CD274-m
CAT# AFG-NVF-100236
Size: 2 μg
726.00 726.0 USD
AffiPLASMID® pGEX-4T-CD274
CAT# AFG-NVF-100235
Size: 2 μg
846.00 846.0 USD
AffiPLASMID® pGEX4T3-YTHDC1
CAT# AFG-NVF-100233
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX4T3-SAV1
CAT# AFG-NVF-100232
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-3-REG4
CAT# AFG-NVF-100231
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX4T3-PKM2
CAT# AFG-NVF-100230
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T3-MIB2
CAT# AFG-NVF-100229
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-3-KDM2A
CAT# AFG-NVF-100228
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T3-ITLN1 (35-313aa) -Y297F
CAT# AFG-NVF-100227
Size: 2 μg
486.00 486.0 USD
486.00 486.0 USD
AffiPLASMID® pGEX-4T3-ITLN1 (35-313aa) -K198L
CAT# AFG-NVF-100225
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T3-ITLN1 (35-313aa)
CAT# AFG-NVF-100224
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-3-GST-RPS6KB1-K99Q
CAT# AFG-NVF-100223
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-3-GST-RPS6KB1
CAT# AFG-NVF-100222
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-3-GST-FAM117B
CAT# AFG-NVF-100221
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-3-GST-CREB1-S133A
CAT# AFG-NVF-100220
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-3-GST-CREB1
CAT# AFG-NVF-100219
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T3-BMP7 (293-431aa) -6×His
CAT# AFG-NVF-100218
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-3 Plasmid
CAT# AFG-NVF-100217
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pGEX-4T-3
CAT# AFG-NVF-100216
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pGEX4T2-USP25-C178S
CAT# AFG-NVF-100215
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX4T2-USP25
CAT# AFG-NVF-100214
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX4T2-GST-AKT19
CAT# AFG-NVF-100213
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-2-DNAJC6 (810-910aa) -b
CAT# AFG-NVF-100212
Size: 2 μg
726.00 726.0 USD
AffiPLASMID® pGEX-4T-2-CDK4-S285D
CAT# AFG-NVF-100211
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-2-CDK4
CAT# AFG-NVF-100210
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-2
CAT# AFG-NVF-100209
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pGEX-4T-1-ZO-1 (1446-1748aa) Plasmid
CAT# AFG-NVF-100208
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T1-ZBED3 Plasmid
CAT# AFG-NVF-100207
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-1-WSB1 Plasmid
CAT# AFG-NVF-100206
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-WRAP53 Plasmid
CAT# AFG-NVF-100205
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-WDR1 Plasmid
CAT# AFG-NVF-100204
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-VWF Plasmid
CAT# AFG-NVF-100203
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-VNN1 Plasmid
CAT# AFG-NVF-100202
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-VAMP2
CAT# AFG-NVF-100201
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-1-VAMP1
CAT# AFG-NVF-100200
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-1-USP39
CAT# AFG-NVF-100199
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-1-TRIM26
CAT# AFG-NVF-100198
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-1-TPK2 Plasmid
CAT# AFG-NVF-100197
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-TPK1 Plasmid
CAT# AFG-NVF-100196
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-SUV420H2 (2-280aa) Plasmid
CAT# AFG-NVF-100195
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-STX1A
CAT# AFG-NVF-100194
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-1-STAT3 Plasmid
CAT# AFG-NVF-100193
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-ST2 (4-328aa) Plasmid
CAT# AFG-NVF-100192
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-SQSTM1 Plasmid
CAT# AFG-NVF-100191
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-SOCS3 (1-225aa) Plasmid
CAT# AFG-NVF-100190
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-SNAP25
CAT# AFG-NVF-100189
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-1-SMYD3 Plasmid
CAT# AFG-NVF-100188
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-RRM2
CAT# AFG-NVF-100187
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-1-RHOA (1-193aa) Plasmid
CAT# AFG-NVF-100186
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-RAF1
CAT# AFG-NVF-100185
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-1-PTPRO Plasmid
CAT# AFG-NVF-100184
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX4T1-PTGES
CAT# AFG-NVF-100183
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T1-PTBP1 Plasmid
CAT# AFG-NVF-100182
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T1-PRMT5 Plasmid
CAT# AFG-NVF-100181
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-1-PRKDC (2801-3500aa) Plasmid
CAT# AFG-NVF-100180
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-PDRG1 Plasmid
CAT# AFG-NVF-100179
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-PDE2 Plasmid
CAT# AFG-NVF-100178
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-OCLN (269-522aa) Plasmid
CAT# AFG-NVF-100177
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-NRDP1 (47-232aa) Plasmid
CAT# AFG-NVF-100176
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-MUC20 (255-555aa) Plasmid
CAT# AFG-NVF-100175
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-MMP9 (1-100aa) Plasmid
CAT# AFG-NVF-100174
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-MAPK1
CAT# AFG-NVF-100173
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-1-MAP2K1
CAT# AFG-NVF-100172
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-1-LMNB1
CAT# AFG-NVF-100171
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-1-LGR5 (23-317aa) Plasmid
CAT# AFG-NVF-100170
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-KEAP1 (325-624aa) Plasmid
CAT# AFG-NVF-100169
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-JTV1 Plasmid
CAT# AFG-NVF-100168
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T-1-IL6 (FL) Plasmid
CAT# AFG-NVF-100167
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pGEX-4T1-IGF2BP1 Plasmid
CAT# AFG-NVF-100166
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pGEX-4T-1-IFIT3 Plasmid
CAT# AFG-NVF-100165
Size: 2 μg
495.60 495.6 USD