pCR Plasmids

pCR plasmids are specialized cloning vectors designed for efficient cloning of PCR products. These vectors are part of the TA (Taq polymerase-based) cloning system and are specifically engineered to simplify the ligation and propagation of DNA fragments generated by PCR. The vectors utilize the single 3′-adenine (A) overhangs added by Taq polymerase to ligate into complementary 3′-thymine (T) overhangs on the plasmid.

Key Features of pCR Plasmids
  • 3′-T Overhangs:
    • Pre-linearized plasmids with single 3′-thymine overhangs on both ends, facilitating direct ligation of PCR products with complementary 3′-A overhangs.
  • Promoter Sites:
    • Typically include dual promoters, such as T7 and SP6, flanking the cloning site for in vitro RNA transcription or bidirectional sequencing.
  • Selectable Markers:
    • Ampicillin Resistance (ampR) or Kanamycin Resistance (kanR): Provides antibiotic resistance for selection in E. coli.
    • LacZα Fragment: Allows blue-white screening for identifying recombinant colonies on X-gal/IPTG plates.
  • Multiple Cloning Site (MCS):
    • Contains restriction sites for downstream manipulation of cloned fragments.
Applications of pCR Plasmids
  • PCR Product Cloning:
    • Designed for direct ligation of PCR-amplified DNA fragments without additional modification steps.
  • Blue-White Screening:
    • Simplifies identification of recombinant colonies using lacZα-based color screening.
  • Sequencing:
    • Used for sequencing PCR products due to the universal primer-binding sites flanking the cloning region.
  • Subcloning:
    • Serve as intermediate vectors for transferring PCR products to other specialized vectors for expression, mutagenesis, or functional studies.

pCR plasmids are versatile and efficient tools for cloning PCR products, providing seamless integration into workflows involving sequencing, subcloning, and RNA synthesis. Their unique 3′-T overhangs, combined with features like blue-white screening and high-copy-number replication, make them indispensable in molecular biology research. 

For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pCRY2PHR-Linker-BAX (human)
CAT# AFG-GNC-13923
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pPCRScript-ZSWIM8
CAT# AFG-NVF-111352
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-ZNF415
CAT# AFG-NVF-111351
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-UBASH3A
CAT# AFG-NVF-111350
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-SSX2B
CAT# AFG-NVF-111349
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-SEC14L3
CAT# AFG-NVF-111348
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-RFPL2
CAT# AFG-NVF-111347
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-RETNLB
CAT# AFG-NVF-111346
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-RANBP10
CAT# AFG-NVF-111345
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-PTTG2
CAT# AFG-NVF-111344
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-PRKAA2
CAT# AFG-NVF-111343
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-PGLYRP3
CAT# AFG-NVF-111342
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-PDE6H
CAT# AFG-NVF-111341
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-PAEP
CAT# AFG-NVF-111340
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-PAAF1
CAT# AFG-NVF-111339
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-NELL1
CAT# AFG-NVF-111338
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-NAT8B
CAT# AFG-NVF-111337
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-MS4A6E
CAT# AFG-NVF-111336
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-MC3R
CAT# AFG-NVF-111335
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-LTB
CAT# AFG-NVF-111334
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-LIM2
CAT# AFG-NVF-111333
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-LEP
CAT# AFG-NVF-111332
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-LAIR2
CAT# AFG-NVF-111331
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-LACRT
CAT# AFG-NVF-111330
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-KLK4
CAT# AFG-NVF-111329
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-KitLG
CAT# AFG-NVF-111328
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-IL20
CAT# AFG-NVF-111327
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-IFNA6
CAT# AFG-NVF-111326
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-HTR2A
CAT# AFG-NVF-111325
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-HRC
CAT# AFG-NVF-111324
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-HBD
CAT# AFG-NVF-111323
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-GAGE2A
CAT# AFG-NVF-111322
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-GAGE12C
CAT# AFG-NVF-111321
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-GAGE1
CAT# AFG-NVF-111320
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-FPR3
CAT# AFG-NVF-111319
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-FABP2
CAT# AFG-NVF-111318
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-CYP4F22
CAT# AFG-NVF-111317
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-CRYBB3
CAT# AFG-NVF-111316
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-CRYBA4
CAT# AFG-NVF-111315
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-CRYBA1
CAT# AFG-NVF-111314
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-CRLS1
CAT# AFG-NVF-111313
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-CNR2
CAT# AFG-NVF-111312
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-CLDN16
CAT# AFG-NVF-111311
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-CHRNB3
CAT# AFG-NVF-111310
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-CER1
CAT# AFG-NVF-111309
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-CELA2B
CAT# AFG-NVF-111308
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-CD200R1
CAT# AFG-NVF-111307
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-CCL18
CAT# AFG-NVF-111306
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-CBLN4
CAT# AFG-NVF-111305
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-CAV3
CAT# AFG-NVF-111304
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-CA8
CAT# AFG-NVF-111303
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCRScript-C19orf73
CAT# AFG-NVF-111302
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCR-Script Amp SK (+) -ZNF415
CAT# AFG-NVF-111301
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCR-Script Amp SK (+) -PGLYRP3
CAT# AFG-NVF-111300
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCR-Script Amp SK (+) -IFNB1
CAT# AFG-NVF-111299
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pPCR-Script Amp SK (+) -IFNA6
CAT# AFG-NVF-111298
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCR-Script Amp SK (+) -CD200R1
CAT# AFG-NVF-111297
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCR-Script Amp SK (+) -CCL18
CAT# AFG-NVF-111296
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pPCR-Script Amp SK (+) -AZU1
CAT# AFG-NVF-111295
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pOTB7-NTPCR
CAT# AFG-NVF-109734
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pET22B, SA, PcrA
CAT# AFG-NVF-096244
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pET22B, PcrA, 2Bchimera
CAT# AFG-NVF-096243
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCRY2PHR-mCherryN1-Linker-BAX
CAT# AFG-NVF-089236
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCRY2PHR-mCherryN1
CAT# AFG-NVF-089235
Size: 2 μg
387.60 387.6 USD
AffiPLASMID® pCRY2PHR-Linker-BAX Plasmid
CAT# AFG-NVF-089234
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-ZNF236
CAT# AFG-NVF-089233
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-ZNF229
CAT# AFG-NVF-089232
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-ZKSCAN2
CAT# AFG-NVF-089231
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-WASHC4
CAT# AFG-NVF-089230
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-USP45
CAT# AFG-NVF-089229
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-USP42
CAT# AFG-NVF-089228
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-TOPBP1 Plasmid
CAT# AFG-NVF-089227
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-TNN
CAT# AFG-NVF-089226
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-TMEM63C
CAT# AFG-NVF-089225
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-TAOK1
CAT# AFG-NVF-089224
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-SPHKAP
CAT# AFG-NVF-089223
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-SOS2
CAT# AFG-NVF-089222
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-SMC1A
CAT# AFG-NVF-089221
Size: 2 μg
846.00 846.0 USD
AffiPLASMID® pCR-XL-TOPO-SLIT1
CAT# AFG-NVF-089220
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-SLC6A20
CAT# AFG-NVF-089219
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-SLC24A1
CAT# AFG-NVF-089218
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-SLC22A3
CAT# AFG-NVF-089217
Size: 2 μg
606.00 606.0 USD
AffiPLASMID® pCR-XL-TOPO-SIPA1L3
CAT# AFG-NVF-089216
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-SHROOM2
CAT# AFG-NVF-089215
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-SETD2
CAT# AFG-NVF-089214
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-SEC31B
CAT# AFG-NVF-089213
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-SEC14L1
CAT# AFG-NVF-089212
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-RPTOR
CAT# AFG-NVF-089211
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-PTPRG
CAT# AFG-NVF-089210
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-PPP1R9A
CAT# AFG-NVF-089209
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-PHRF1
CAT# AFG-NVF-089208
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-OR4F4-4
CAT# AFG-NVF-089207
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-OR4F4
CAT# AFG-NVF-089206
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pCR-XL-TOPO-OR2AT4
CAT# AFG-NVF-089205
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-OR1L8
CAT# AFG-NVF-089204
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-OR10AG1
CAT# AFG-NVF-089203
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-NRAP
CAT# AFG-NVF-089202
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pCR-XL-TOPO-NPC1L1
CAT# AFG-NVF-089201
Size: 2 μg
428.40 428.40000000000003 USD
AffiPLASMID® pCR-XL-TOPO-NCOA6
CAT# AFG-NVF-089200
Size: 2 μg
486.00 486.0 USD