pCMV Plasmids
pCMV plasmids are versatile expression vectors designed for robust and high-level gene expression in mammalian cells. These plasmids utilize the cytomegalovirus (CMV) immediate-early promoter, which is one of the strongest and most widely used constitutive promoters in mammalian systems. pCMV plasmids are extensively employed in molecular biology, biomedical research, and therapeutic development for gene function studies, protein production, and other applications.
Key Features of pCMV Plasmids
- CMV Promoter:
- Drives constitutive, high-level expression in a wide range of mammalian cells.
- Multiple Cloning Site (MCS):
- Contains multiple unique restriction enzyme sites for flexible insertion of the gene of interest.
- Selectable Markers:
- Antibiotic Resistance: Ampicillin (ampR) for bacterial selection.
- Some variants also include mammalian selection markers such as neomycin (G418 resistance) or hygromycin resistance.
- Replication Origin (Ori):
- Derived from ColE1, supporting high-copy-number replication in E. coli.
- Optional Tags:
- Variants often include fusion tags such as His6, FLAG, GFP, or Myc for protein detection, tracking, or purification.
Applications of pCMV Plasmids
- Gene Overexpression:
- Introduce and overexpress genes to study their function in mammalian cells.
- Protein Production:
- Produce recombinant proteins for structural, functional, or therapeutic studies.
- Transfection Studies:
- Optimize transfection protocols using the strong CMV promoter for high expression levels.
- Reporter Assays:
- Study promoter activity, protein localization, or gene regulation using tagged variants or reporter genes.
- Therapeutic Research:
- Used for DNA-based vaccines and gene therapy preclinical studies.
pCMV plasmids are powerful and versatile tools for high-level gene expression in mammalian cells. Their strong CMV promoter, customizable design, and broad applications make them a cornerstone in molecular biology, protein production, and therapeutic research. Whether for transient transfection or stable expression, pCMV plasmids provide reliable performance across diverse experimental settings.
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