pcDNA Plasmids are a family of mammalian expression vectors widely used in molecular biology for the transient or stable expression of recombinant proteins in mammalian cells. Developed by Invitrogen (now part of Thermo Fisher Scientific), these plasmids are designed to provide high levels of protein expression driven by the strong human cytomegalovirus (CMV) immediate-early promoter.

Content

pcDNA Plasmids: Technical Insights

  • Structure and Components:
    • Origin of Replication (ori): pcDNA plasmids contain the ColE1 origin of replication for propagation in Escherichia coli, enabling high plasmid yields for transfection experiments.
    • Antibiotic Resistance Gene:
      • ampR: Provides resistance to ampicillin, allowing for the selection of bacteria that have taken up the plasmid.
    • CMV Promoter: The human CMV immediate-early promoter drives high-level expression of the gene of interest in mammalian cells. This promoter is strong and constitutive, ensuring robust expression across various cell types.
    • Multiple Cloning Site (MCS): The MCS contains several unique restriction enzyme sites, facilitating the insertion of the gene of interest. The site is positioned downstream of the CMV promoter for efficient transcription.
    • Polyadenylation Signal (polyA): A synthetic bovine growth hormone (BGH) polyadenylation signal downstream of the MCS ensures proper mRNA processing and stability.
    • SV40 Origin: The SV40 origin of replication is included to allow for episomal replication in cells expressing the SV40 large T antigen, such as COS-7 cells, enhancing plasmid copy number and expression.
    • Neomycin Resistance Gene (neoR): Provides resistance to G418 (geneticin) in mammalian cells, allowing for the selection of stably transfected cells.
  • Applications:
    • Protein Expression: pcDNA plasmids are primarily used for the expression of recombinant proteins in mammalian cells. The strong CMV promoter ensures high levels of expression, suitable for functional studies, protein-protein interaction assays, and biochemical analyses.
    • Gene Function Studies: By expressing wild-type or mutant genes, researchers can study the effects on cellular pathways, identify gene
AffiPLASMID® pCDNA-Ikkb-FLAG WT
CAT# AFG-GNC-3539
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pCDNA-Ikka-HA WT
CAT# AFG-GNC-3538
Size: 1 Unit
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AffiPLASMID® pcDNA-GalNAc-T2-GFP-ESCargo (FTV)
CAT# AFG-GNC-3537
Size: 1 Unit
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AffiPLASMID® pCDNA6-V5-His-CaV1.2-m
CAT# AFG-GNC-3536
Size: 1 Unit
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AffiPLASMID® pCDNA6-HA-AML1-ETO
CAT# AFG-GNC-3535
Size: 1 Unit
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AffiPLASMID® pCDNA6-FLAG-CBX7
CAT# AFG-GNC-3534
Size: 1 Unit
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AffiPLASMID® pCDNA5-TBXA2R (human)
CAT# AFG-GNC-3531
Size: 1 Unit
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AffiPLASMID® pCDNA5-FRT-To-GNA15 (human) -Hyg
CAT# AFG-GNC-3530
Size: 1 Unit
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AffiPLASMID® pCDNA5FRT-FLAG-OGT1
CAT# AFG-GNC-3529
Size: 1 Unit
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AffiPLASMID® pCDNA5-FLAG-ISG20 (human) -Hyg
CAT# AFG-GNC-3528
Size: 1 Unit
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AffiPLASMID® pCDNA5-3×FLAG-PRMT9 (human) -Hyg
CAT# AFG-GNC-3527
Size: 1 Unit
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AffiPLASMID® pCDNA5-3×FLAG-PRMT8 (human) -Hyg
CAT# AFG-GNC-3526
Size: 1 Unit
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AffiPLASMID® pCDNA5-3×FLAG-PRMT7 (human) -Hyg
CAT# AFG-GNC-3525
Size: 1 Unit
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AffiPLASMID® pCDNA5-3×FLAG-PRMT6 (human) -Hyg
CAT# AFG-GNC-3524
Size: 1 Unit
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AffiPLASMID® pCDNA5-3×FLAG-PRMT5 (human) -Hyg
CAT# AFG-GNC-3523
Size: 1 Unit
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AffiPLASMID® pCDNA5-3×FLAG-PRMT3 (human) -Hyg
CAT# AFG-GNC-3522
Size: 1 Unit
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AffiPLASMID® pCDNA5-3×FLAG-PRMT2 (human) -Hyg
CAT# AFG-GNC-3521
Size: 1 Unit
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AffiPLASMID® pCDNA5-3×FLAG-PRMT1 (human) -Hyg
CAT# AFG-GNC-3520
Size: 1 Unit
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AffiPLASMID® pCDNA5-3×FLAG-MCS-Hyg
CAT# AFG-GNC-3519
Size: 1 Unit
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AffiPLASMID® pcDNA5 FRT/TO SF-HERC2 (ShB-R)
CAT# AFG-GNC-3515
Size: 1 Unit
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AffiPLASMID® pCDNA4-SAR1B (human) -V5-6×His
CAT# AFG-GNC-3514
Size: 1 Unit
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AffiPLASMID® pCDNA4-PLIN5 (human) -V5-6×His
CAT# AFG-GNC-3513
Size: 1 Unit
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AffiPLASMID® pCDNA4-NRP1 (human) -1-V5-6×His
CAT# AFG-GNC-3512
Size: 1 Unit
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AffiPLASMID® pCDNA4-F8 (human)
CAT# AFG-GNC-3510
Size: 1 Unit
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AffiPLASMID® pCDNA4-CCDC25 (human) -6×His
CAT# AFG-GNC-3509
Size: 1 Unit
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AffiPLASMID® pCDNA3β-FLAG-CBP-HA
CAT# AFG-GNC-3508
Size: 1 Unit
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AffiPLASMID® pcDNA3-TORCAR
CAT# AFG-GNC-3507
Size: 1 Unit
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AffiPLASMID® pCDNA3-TLR9-YFP
CAT# AFG-GNC-3506
Size: 1 Unit
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AffiPLASMID® pCDNA3-TLR4-His
CAT# AFG-GNC-3505
Size: 1 Unit
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AffiPLASMID® pCDNA3-TLR4-3×FLAG
CAT# AFG-GNC-3504
Size: 1 Unit
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AffiPLASMID® pCDNA3-PTGER2-EGFP
CAT# AFG-GNC-3503
Size: 1 Unit
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AffiPLASMID® pCDNA3-mTOR
CAT# AFG-GNC-3501
Size: 1 Unit
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AffiPLASMID® pcDNA3-Lyn-ICUE3
CAT# AFG-GNC-3500
Size: 1 Unit
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AffiPLASMID® pCDNA3-H-Ras_V12
CAT# AFG-GNC-3499
Size: 1 Unit
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AffiPLASMID® pCDNA3-HA-p53
CAT# AFG-GNC-3498
Size: 1 Unit
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AffiPLASMID® pcDNA3-HA-mRipk3
CAT# AFG-GNC-3497
Size: 1 Unit
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AffiPLASMID® pCDNA3-HA-FLAG-RBP2
CAT# AFG-GNC-3496
Size: 1 Unit
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AffiPLASMID® pCDNA3-HA-CTNNB1
CAT# AFG-GNC-3495
Size: 1 Unit
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AffiPLASMID® pCDNA3-HA2-Keap1
CAT# AFG-GNC-3494
Size: 1 Unit
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AffiPLASMID® pCDNA3-FLAG-UbvG08-I44A-deltaGG
CAT# AFG-GNC-3493
Size: 1 Unit
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AffiPLASMID® pcDNA3-FLAG-p53
CAT# AFG-GNC-3492
Size: 1 Unit
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AffiPLASMID® pCDNA3-FLAG-mTOR
CAT# AFG-GNC-3491
Size: 1 Unit
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AffiPLASMID® pCDNA3-FLAG-MAGI1c-m
CAT# AFG-GNC-3490
Size: 1 Unit
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AffiPLASMID® pCDNA3-FLAG-DHX36
CAT# AFG-GNC-3489
Size: 1 Unit
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AffiPLASMID® pCDNA3-FLAG MKK6
CAT# AFG-GNC-3488
Size: 1 Unit
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AffiPLASMID® pCDNA3-EGFP-C4-Nrf2
CAT# AFG-GNC-3487
Size: 1 Unit
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AffiPLASMID® pCDNA3-EGFP-Ash2L
CAT# AFG-GNC-3486
Size: 1 Unit
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AffiPLASMID® pCDNA3-Ash2L
CAT# AFG-GNC-3485
Size: 1 Unit
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AffiPLASMID® pCDNA3/HA-SUMO3 (Sentrin 2)
CAT# AFG-GNC-3484
Size: 1 Unit
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AffiPLASMID® pCDNA3.4-TCF3 (rat) -3×FLAG
CAT# AFG-GNC-3483
Size: 1 Unit
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AffiPLASMID® pCDNA3.4-SREBF1 (rat) -3×FLAG
CAT# AFG-GNC-3482
Size: 1 Unit
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AffiPLASMID® pCDNA3.4-SLC45A4 (human) -3×FLAG
CAT# AFG-GNC-3481
Size: 1 Unit
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AffiPLASMID® pCDNA3.4-SLC45A1 (human) -3×FLAG
CAT# AFG-GNC-3480
Size: 1 Unit
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AffiPLASMID® pCDNA3.4-SIX5 (human) -3×FLAG
CAT# AFG-GNC-3479
Size: 1 Unit
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AffiPLASMID® pcDNA3.4-SIGMAR1 (mouse) -3×FLAG
CAT# AFG-GNC-3478
Size: 1 Unit
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AffiPLASMID® pCDNA3.4-Runx2 (rat) -3×FLAG
CAT# AFG-GNC-3476
Size: 1 Unit
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AffiPLASMID® pCDNA3.4-NRP1 (mouse) -3×FLAG
CAT# AFG-GNC-3475
Size: 1 Unit
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AffiPLASMID® pcDNA3.4-ATP2A2 (mouse) -3×Myc
CAT# AFG-GNC-3474
Size: 1 Unit
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AffiPLASMID® pCDNA3.4-ATF6 (mouse) -3×FLAG
CAT# AFG-GNC-3473
Size: 1 Unit
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AffiPLASMID® pCDNA3.4-AHR (mouse) -3×Myc
CAT# AFG-GNC-3471
Size: 1 Unit
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AffiPLASMID® pCDNA3.4-6×His-UBE2L6 (human)
CAT# AFG-GNC-3470
Size: 1 Unit
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AffiPLASMID® pCDNA3.4-6×His-UBE1L (human)
CAT# AFG-GNC-3469
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-Zeo-GPR88
CAT# AFG-GNC-3467
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-Zeo-GPR35-r
CAT# AFG-GNC-3466
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-Zeo-GPR35-m
CAT# AFG-GNC-3465
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-Zeo-GPR35
CAT# AFG-GNC-3464
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-ZBTB1
CAT# AFG-GNC-3461
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-YY1 (mouse) -3×HA
CAT# AFG-GNC-3460
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-YWHAE (human) -3×FLAG
CAT# AFG-GNC-3459
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-YTHDF1-3×FLAG
CAT# AFG-GNC-3458
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-YAP1-3×FLAG
CAT# AFG-GNC-3457
Size: 1 Unit
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AffiPLASMID® pcDNA3.1-WT-VWF
CAT# AFG-GNC-3456
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-WNT3A (human)
CAT# AFG-GNC-3455
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-VSIR (mouse) -SV40-Neo
CAT# AFG-GNC-3454
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-VEGFA
CAT# AFG-GNC-3453
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-V5-HisB-MARK2 (human)
CAT# AFG-GNC-3450
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-V5-FLAG-USP22
CAT# AFG-GNC-3449
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-USP4L172A-3×Linker-GST
CAT# AFG-GNC-3446
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-USP4K171A-3×linker-GST
CAT# AFG-GNC-3445
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-USP4-AA-3×Linker-GST
CAT# AFG-GNC-3444
Size: 1 Unit
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AffiPLASMID® pCDNA3.1-USP44-3×FLAG
CAT# AFG-GNC-3443
Size: 1 Unit
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