pBluescript Plasmids

pBluescript Plasmids are commonly used vectors in molecular biology for cloning and expression experiments. They are derivatives of the original pUC plasmid vector system, modified to include additional features for various applications.

Key Features

  • Origin of Replication (ori): The plasmid contains the pUC ori which allows for high copy number replication in E. coli, typically producing 100-300 copies per cell.
  • Selectable Marker: The vector includes an ampicillin resistance gene (bla), providing antibiotic resistance to ampicillin. This marker allows for the selection of successfully transformed cells in the presence of ampicillin.
  • Multiple Cloning Site (MCS): The MCS, located within the lacZα gene, provides multiple unique restriction enzyme sites for the insertion of foreign DNA. This site is strategically placed to facilitate cloning of DNA fragments while minimizing disruption to the vector’s essential functions.
  • LacZα Complementation: The plasmid carries a portion of the lacZ gene (lacZα). Insertion of a DNA fragment into the MCS disrupts the lacZα gene, which can be used for blue/white screening. Cells with an insert appear white, while cells without an insert appear blue when grown on X-gal and IPTG media.
  • Promoter Elements: pBluescript vectors typically include strong promoter elements such as the T7 and T3 promoters for transcriptional initiation, allowing for in vitro transcription applications.
  • T7 and T3 Transcriptional Control: The plasmid has T7 and T3 RNA polymerase promoters flanking the MCS, facilitating high-yield transcription of cloned genes in systems where T7 or T3 polymerases are available.
  • Size and Features: The plasmid backbone is generally about 3.0-3.5 kb in length, with features designed to ensure ease of use and versatility in cloning and expression applications.

Content:

  • Backbone: ~3.0-3.5 kb plasmid DNA.
  • Genes:
    • Ampicillin resistance gene (bla).
    • LacZα fragment.
  • Promoters:
    • T7 promoter.
    • T3 promoter.
  • Multiple Cloning Site (MCS): Contains various restriction sites for insertion.

Applications:

  • Cloning: Facilitates the insertion of DNA fragments for cloning purposes.
  • Expression Studies: Useful for studying gene expression under the control of T7 or T3 promoters.
  • Blue/White Screening: Enables differentiation of recombinant colonies from non-recombinant colonies.

AffiPLASMID® pBluescriptR-ZZZ3
CAT# AFG-NVF-075619
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-ZXDA
CAT# AFG-NVF-075618
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-ZPLD1
CAT# AFG-NVF-075615
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-ZNRF4
CAT# AFG-NVF-075614
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-ZNF84
CAT# AFG-NVF-075610
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-ZNF775
CAT# AFG-NVF-075608
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-ZNF771 Plasmid
CAT# AFG-NVF-075607
Size: 2 μg
409.20 409.2 USD
AffiPLASMID® pBluescriptR-ZNF714
CAT# AFG-NVF-075605
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-ZNF677
CAT# AFG-NVF-075603
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-ZNF555
CAT# AFG-NVF-075593
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-ZNF550
CAT# AFG-NVF-075592
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-ZNF502
CAT# AFG-NVF-075586
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-ZNF48
CAT# AFG-NVF-075585
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-ZNF451
CAT# AFG-NVF-075583
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-ZNF439
CAT# AFG-NVF-075581
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-ZNF365
CAT# AFG-NVF-075576
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-ZMYND12
CAT# AFG-NVF-075556
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-ZMYM2 Plasmid
CAT# AFG-NVF-075555
Size: 2 μg
409.20 409.2 USD
AffiPLASMID® pBluescriptR-ZC3H3
CAT# AFG-NVF-075538
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-ZC3H14
CAT# AFG-NVF-075537
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-YY1AP1
CAT# AFG-NVF-075532
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-YTHDF1
CAT# AFG-NVF-075529
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-YPEL5
CAT# AFG-NVF-075528
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-YIF1B
CAT# AFG-NVF-075525
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-YBX1
CAT# AFG-NVF-075524
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-WDR20
CAT# AFG-NVF-075505
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-WDR11
CAT# AFG-NVF-075504
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-VWA3B
CAT# AFG-NVF-075498
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-VTA1 Plasmid
CAT# AFG-NVF-075496
Size: 2 μg
409.20 409.2 USD
AffiPLASMID® pBlueScriptR-VSTM2A
CAT# AFG-NVF-075494
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-VPS54
CAT# AFG-NVF-075490
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-USPL1
CAT# AFG-NVF-075480
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-USP6NL Plasmid
CAT# AFG-NVF-075479
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-USP44
CAT# AFG-NVF-075478
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBlueScriptR-USP38
CAT# AFG-NVF-075477
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-USP16
CAT# AFG-NVF-075474
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-UQCRB
CAT# AFG-NVF-075472
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-UBL3
CAT# AFG-NVF-075454
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-UBE2Q2P2
CAT# AFG-NVF-075452
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TSEN15 Plasmid
CAT# AFG-NVF-075416
Size: 2 μg
409.20 409.2 USD
AffiPLASMID® pBlueScriptR-TSEN15
CAT# AFG-NVF-075415
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBlueScriptR-TSC1
CAT# AFG-NVF-075414
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TRPM3 Plasmid
CAT# AFG-NVF-075413
Size: 2 μg
409.20 409.2 USD
AffiPLASMID® pBluescriptR-TRMT10B
CAT# AFG-NVF-075412
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-TRIM45
CAT# AFG-NVF-075411
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-TRIM27
CAT# AFG-NVF-075407
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-TRIM24
CAT# AFG-NVF-075406
Size: 2 μg
387.60 387.6 USD
AffiPLASMID® pBlueScriptR-TPD52L3
CAT# AFG-NVF-075396
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-TP53I11
CAT# AFG-NVF-075394
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-TMX1
CAT# AFG-NVF-075386
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TMPRSS12
CAT# AFG-NVF-075385
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-TMEM74
CAT# AFG-NVF-075384
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TMEM270
CAT# AFG-NVF-075380
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TMEM180
CAT# AFG-NVF-075371
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TMEM154
CAT# AFG-NVF-075365
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-TMEM146
CAT# AFG-NVF-075364
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TMEM106B
CAT# AFG-NVF-075358
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TMC5
CAT# AFG-NVF-075352
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TLE4
CAT# AFG-NVF-075348
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-TLE3
CAT# AFG-NVF-075347
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-TKTL2
CAT# AFG-NVF-075346
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBlueScriptR-TKTL1
CAT# AFG-NVF-075345
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-TJP2
CAT# AFG-NVF-075344
Size: 2 μg
387.60 387.6 USD
AffiPLASMID® pBluescriptR-TIPARP
CAT# AFG-NVF-075343
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-THEG Plasmid
CAT# AFG-NVF-075339
Size: 2 μg
409.20 409.2 USD
AffiPLASMID® pBluescriptR-THAP3
CAT# AFG-NVF-075338
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-THAP1
CAT# AFG-NVF-075337
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TGIF1
CAT# AFG-NVF-075336
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TGFBR2
CAT# AFG-NVF-075335
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-Tex36 Plasmid
CAT# AFG-NVF-075332
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TEX35 Plasmid
CAT# AFG-NVF-075331
Size: 2 μg
409.20 409.2 USD
AffiPLASMID® pBlueScriptR-TEX30
CAT# AFG-NVF-075330
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-TEX2
CAT# AFG-NVF-075329
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TEX11
CAT# AFG-NVF-075326
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TERB2
CAT# AFG-NVF-075325
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-TENT5D
CAT# AFG-NVF-075324
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-TENT2
CAT# AFG-NVF-075323
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TEKT4 Plasmid
CAT# AFG-NVF-075322
Size: 2 μg
409.20 409.2 USD
AffiPLASMID® pBlueScriptR-TEKT1
CAT# AFG-NVF-075321
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-TEF
CAT# AFG-NVF-075320
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-TDRD3
CAT# AFG-NVF-075319
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-TCTEX1D4
CAT# AFG-NVF-075316
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TCERG1L
CAT# AFG-NVF-075314
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-TC2N
CAT# AFG-NVF-075312
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-TBC1D15
CAT# AFG-NVF-075309
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-SYT9
CAT# AFG-NVF-075302
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-SYNDIG1
CAT# AFG-NVF-075300
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBlueScriptR-SYCP1
CAT# AFG-NVF-075299
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBlueScriptR-SV2B
CAT# AFG-NVF-075296
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBlueScriptR-SUSD3
CAT# AFG-NVF-075294
Size: 2 μg
344.40 344.40000000000003 USD
AffiPLASMID® pBluescriptR-SUN3
CAT# AFG-NVF-075290
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-SUMO1
CAT# AFG-NVF-075288
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-SUCLG2
CAT# AFG-NVF-075286
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-SUCLA2 Plasmid
CAT# AFG-NVF-075285
Size: 2 μg
409.20 409.2 USD
AffiPLASMID® pBlueScriptR-STK38L
CAT# AFG-NVF-075281
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-STK36
CAT# AFG-NVF-075280
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-STAU1
CAT# AFG-NVF-075275
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-STAM2
CAT# AFG-NVF-075272
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBlueScriptR-SSMEM1
CAT# AFG-NVF-075267
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pBluescriptR-SREK1
CAT# AFG-NVF-075259
Size: 2 μg
486.00 486.0 USD