Overview of Ubiquitin Plasmids

Ubiquitin Plasmids are genetic constructs used in molecular biology to study the ubiquitin-proteasome system, protein degradation, and post-translational modifications. These plasmids typically contain the coding sequence for ubiquitin, a small regulatory protein found in almost all eukaryotic cells, which tags proteins for degradation.

Key Features

  • Ubiquitin Gene: The plasmid includes the ubiquitin gene sequence, often with promoters and regulatory elements to ensure proper expression in host cells.
  • Promoters: Strong promoters (e.g., CMV, EF1α) drive high-level expression of ubiquitin, facilitating the study of its effects on target proteins.
  • Selectable Markers: Antibiotic resistance genes (e.g., ampicillin, kanamycin) enable selection of successfully transformed cells.
  • Multiple Cloning Sites (MCS): Allow the insertion of genes of interest that can be tagged or modified by ubiquitin.
  • Tagging: Some plasmids encode ubiquitin fusion proteins, where ubiquitin is fused to another protein, aiding in studying ubiquitination and proteasomal degradation.

Applications

  • Protein Degradation Studies: Investigate how proteins are tagged with ubiquitin and degraded by the proteasome.
  • Post-Translational Modifications: Study ubiquitin's role in modifying protein function, localization, and interactions.
  • Gene Regulation: Explore how ubiquitination affects transcription factors and other regulatory proteins.
  • Disease Models: Model diseases related to ubiquitin-proteasome system dysfunction, such as neurodegenerative disorders and cancers.

Methodology

  • Cloning: Insert the ubiquitin gene or ubiquitin fusion constructs into the plasmid vector using restriction enzymes and ligation.
  • Transformation: Introduce the plasmid into bacterial cells for propagation and amplification.
  • Transfection: Transfer the plasmid into eukaryotic cells to express ubiquitin and study its effects.
  • Analysis: Use techniques like Western blotting, immunoprecipitation, and fluorescence microscopy to analyze ubiquitinated proteins.
AffiPLASMID® pRK5-HA-Ubiquitin-WT
CAT# AFG-GNC-25016
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-KO
CAT# AFG-GNC-25015
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K6R
CAT# AFG-GNC-25014
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K63R
CAT# AFG-GNC-25013
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K63
CAT# AFG-GNC-25012
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K6
CAT# AFG-GNC-25011
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K48R
CAT# AFG-GNC-25010
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K48
CAT# AFG-GNC-25009
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K33R
CAT# AFG-GNC-25008
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K33
CAT# AFG-GNC-25007
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K29R
CAT# AFG-GNC-25006
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K29
CAT# AFG-GNC-25005
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K27R
CAT# AFG-GNC-25004
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K27
CAT# AFG-GNC-25003
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K11R
CAT# AFG-GNC-25002
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K11
CAT# AFG-GNC-25001
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-6×His-Ubiquitin-K48
CAT# AFG-GNC-24990
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pLV3-CMV-Ubiquitin (human) -HA-Blast
CAT# AFG-GNC-21472
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pEBB-BT-Ubiquitin
CAT# AFG-GNC-14026
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pCDNA3.1-6×His-Ubiquitin-K63R
CAT# AFG-GNC-2120
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pCDNA3.1-6×His-Ubiquitin-K48R
CAT# AFG-GNC-2119
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® HA-Ubiquitin
CAT# AFG-GNC-0101
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K29 Plasmid
CAT# AFG-NVF-111850
Size: 2 μg
276.00 276.0 USD
AffiPLASMID® pRK5-6×His-Ubiquitin-K48 Plasmid
CAT# AFG-NVF-111842
Size: 2 μg
276.00 276.0 USD
AffiPLASMID® pRK5- HA- Ubiquitin- WT
CAT# AFG-NVF-111840
Size: 2 μg
182.90 182.9 USD
AffiPLASMID® pRK5- HA- Ubiquitin- K48
CAT# AFG-NVF-111839
Size: 2 μg
182.90 182.9 USD
390.00 390.0 USD
276.00 276.0 USD
AffiPLASMID® pmCherry-HA-6XHis-Ubiquitin-WT
CAT# AFG-NVF-104801
Size: 2 μg
276.00 276.0 USD
AffiPLASMID® pmCherry-HA-6XHis-Ubiquitin-K63R
CAT# AFG-NVF-104800
Size: 2 μg
276.00 276.0 USD
AffiPLASMID® pmCherry-HA-6XHis-Ubiquitin-K63
CAT# AFG-NVF-104799
Size: 2 μg
276.00 276.0 USD
AffiPLASMID® pmCherry-HA-6XHis-Ubiquitin-K48R
CAT# AFG-NVF-104798
Size: 2 μg
276.00 276.0 USD
AffiPLASMID® pmCherry-HA-6XHis-Ubiquitin-K48
CAT# AFG-NVF-104797
Size: 2 μg
276.00 276.0 USD
AffiPLASMID® pEBB- BT- Ubiquitin
CAT# AFG-NVF-091300
Size: 2 μg
217.10 217.1 USD
AffiPLASMID® pCMV-myc-Ubiquitin (WT) Plasmid
CAT# AFG-NVF-078658
Size: 2 μg
283.60 283.6 USD
AffiPLASMID® pCMV-myc-Ubiquitin (K48R) Plasmid
CAT# AFG-NVF-078657
Size: 2 μg
283.60 283.6 USD
AffiPLASMID® pCMV-Myc-Ubiquitin (K11R) Plasmid
CAT# AFG-NVF-078656
Size: 2 μg
283.60 283.6 USD
AffiPLASMID® pcDNA3.1-HA-Ubiquitin Plasmid
CAT# AFG-NVF-077256
Size: 2 μg
283.60 283.6 USD
AffiPLASMID® pCDNA3.1-6×His-Ubiquitin-wt
CAT# AFG-NVF-076910
Size: 2 μg
276.00 276.0 USD
276.00 276.0 USD
276.00 276.0 USD