Ubiquitin Plasmids

Ubiquitin plasmids are specialized genetic tools used in molecular biology to study protein ubiquitination, post-translational modifications, and protein degradation pathways. Ubiquitin is a small regulatory protein found in almost all eukaryotic cells and is involved in tagging proteins for degradation via the proteasome. Ubiquitin plasmids allow researchers to manipulate the ubiquitination process for various applications, such as understanding cellular processes or designing experiments to study protein stability.

Content of Ubiquitin Plasmids
  • Ubiquitin-Encoding Sequence:
    • Contains the coding sequence for ubiquitin, often with codon optimization for specific expression systems (e.g., human, yeast, or bacterial cells).
  • Promoter Region:
    • A strong, constitutive or inducible promoter (e.g., CMV, T7, or Gal1) ensures the controlled expression of ubiquitin or ubiquitin-fusion proteins.
  • Tagging Sequences:
    • Fusion tags such as GFP, HA, or His6 may be included for easy detection and purification of ubiquitin or ubiquitinated proteins.
  • Multiple Cloning Sites (MCS):
    • Provide flexibility for inserting target genes that require ubiquitin tagging.
Applications of Ubiquitin Plasmids
  • Protein Ubiquitination Studies:
    • Ubiquitin plasmids are used to study the ubiquitination of specific target proteins by co-expressing ubiquitin and the target in cell lines.
    • Researchers often analyze the effects of mutations in ubiquitin or target proteins on ubiquitination efficiency.
  • Proteasomal Degradation:
    • These plasmids help investigate how ubiquitination targets proteins for proteasomal degradation, aiding in drug discovery for diseases like cancer or neurodegeneration.
  • Protein Stability Assays:
    • Fusion of ubiquitin to proteins allows controlled degradation to study protein turnover and stability.
  • Pathway Analysis:
    • Enables the identification of E1, E2, and E3 enzymes responsible for ubiquitination and their role in signaling pathways.

By providing a modular and customizable platform, ubiquitin plasmids have become indispensable tools for studying the ubiquitin-proteasome pathway and its implications in cellular regulation and disease.

AffiPLASMID® pRK5-HA-Ubiquitin-WT
CAT# AFG-GNC-25016
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-KO
CAT# AFG-GNC-25015
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K6R
CAT# AFG-GNC-25014
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K63R
CAT# AFG-GNC-25013
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K63
CAT# AFG-GNC-25012
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K6
CAT# AFG-GNC-25011
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K48R
CAT# AFG-GNC-25010
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K48
CAT# AFG-GNC-25009
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K33R
CAT# AFG-GNC-25008
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K33
CAT# AFG-GNC-25007
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K29R
CAT# AFG-GNC-25006
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K29
CAT# AFG-GNC-25005
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K27R
CAT# AFG-GNC-25004
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K27
CAT# AFG-GNC-25003
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K11R
CAT# AFG-GNC-25002
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K11
CAT# AFG-GNC-25001
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-6×His-Ubiquitin-K48
CAT# AFG-GNC-24990
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pLV3-CMV-Ubiquitin (human) -HA-Blast
CAT# AFG-GNC-21472
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pEBB-BT-Ubiquitin
CAT# AFG-GNC-14026
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pCDNA3.1-6×His-Ubiquitin-K63R
CAT# AFG-GNC-2120
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pCDNA3.1-6×His-Ubiquitin-K48R
CAT# AFG-GNC-2119
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® HA-Ubiquitin
CAT# AFG-GNC-0101
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pRK5-HA-Ubiquitin-K29 Plasmid
CAT# AFG-NVF-111850
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pRK5-6×His-Ubiquitin-K48 Plasmid
CAT# AFG-NVF-111842
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pRK5- HA- Ubiquitin- WT
CAT# AFG-NVF-111840
Size: 2 μg
368.40 368.40000000000003 USD
AffiPLASMID® pRK5- HA- Ubiquitin- K48
CAT# AFG-NVF-111839
Size: 2 μg
368.40 368.40000000000003 USD
630.00 630.0 USD
486.00 486.0 USD
AffiPLASMID® pmCherry-HA-6XHis-Ubiquitin-WT
CAT# AFG-NVF-104801
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pmCherry-HA-6XHis-Ubiquitin-K63R
CAT# AFG-NVF-104800
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pmCherry-HA-6XHis-Ubiquitin-K63
CAT# AFG-NVF-104799
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pmCherry-HA-6XHis-Ubiquitin-K48R
CAT# AFG-NVF-104798
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pmCherry-HA-6XHis-Ubiquitin-K48
CAT# AFG-NVF-104797
Size: 2 μg
486.00 486.0 USD
AffiPLASMID® pEBB- BT- Ubiquitin
CAT# AFG-NVF-091300
Size: 2 μg
411.60 411.6 USD
AffiPLASMID® pCMV-myc-Ubiquitin (WT) Plasmid
CAT# AFG-NVF-078658
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pCMV-myc-Ubiquitin (K48R) Plasmid
CAT# AFG-NVF-078657
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pCMV-Myc-Ubiquitin (K11R) Plasmid
CAT# AFG-NVF-078656
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pcDNA3.1-HA-Ubiquitin Plasmid
CAT# AFG-NVF-077256
Size: 2 μg
495.60 495.6 USD
AffiPLASMID® pCDNA3.1-6×His-Ubiquitin-wt
CAT# AFG-NVF-076910
Size: 2 μg
486.00 486.0 USD
486.00 486.0 USD
486.00 486.0 USD