TNFRSF10B Plasmids

TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily Member 10B), also known as TRAIL-R2 (TRAIL receptor 2) or DR5 (Death Receptor 5), is a receptor that binds TRAIL (TNF-related apoptosis-inducing ligand). Upon activation, TNFRSF10B recruits adaptor proteins and caspases to form the death-inducing signaling complex (DISC), initiating apoptosis. TNFRSF10B is a critical component of the extrinsic apoptotic pathway and is also involved in non-apoptotic signaling, including cell survival and immune regulation. 

Content of TNFRSF10B Plasmids

• TNFRSF10B Coding Sequence:
  • Encodes the full-length TNFRSF10B protein, including its extracellular TRAIL-binding domain, transmembrane domain, and intracellular death domain.
  • Mutant forms of TNFRSF10B may include truncated versions lacking the death domain or specific point mutations to disrupt function.
• Promoter:
  • Constitutive Promoters: (e.g., CMV, EF1α) for robust expression.
  • Inducible Promoters: (e.g., tetracycline-inducible systems) for regulated expression.
  • Tissue-Specific Promoters: For targeted expression in specific cell types, such as immune or tumor cells.
• Tagging Sequences:
  • Fusion tags (e.g., FLAG, GFP, or His6) for protein tracking, visualization, and purification.
• Selectable Markers:
  • Antibiotic resistance genes (e.g., ampicillin, kanamycin) for plasmid selection in bacterial or mammalian systems.

Applications of TNFRSF10B Plasmids

• Apoptosis Research:
  • Study the molecular mechanisms of TRAIL-induced apoptosis, including DISC assembly and downstream caspase activation.
• Cancer Biology:
  • Investigate TNFRSF10B's role in tumor progression, resistance to TRAIL-based therapies, and its potential as a therapeutic target.
  • Explore how TNFRSF10B expression and function differ in cancerous versus normal cells.
• Therapeutic Screening:
  • Use TNFRSF10B plasmids to screen for small molecules, TRAIL variants, or antibodies that enhance or inhibit its apoptotic signaling.
• Resistance Mechanism Studies:
  • Explore mechanisms by which cancer cells evade TNFRSF10B-mediated apoptosis, such as receptor downregulation, mutations, or interference by decoy receptors.

TNFRSF10B plasmids are essential tools for studying TRAIL-mediated apoptosis, immune regulation, and cancer therapy development. They provide insights into the molecular mechanisms of death receptor signaling and its implications in cancer resistance, making them invaluable in both basic and translational research.