TNFRSF10A Plasmids

TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily Member 10A), also known as TRAIL-R1 (TRAIL receptor 1), is a death receptor that binds TRAIL (TNF-related apoptosis-inducing ligand). TNFRSF10A initiates apoptosis upon ligand binding by recruiting and activating caspases through the death-inducing signaling complex (DISC). 

Content of TNFRSF10A Plasmids

• TNFRSF10A Coding Sequence:
  • Encodes the full-length TNFRSF10A protein, including extracellular ligand-binding domains, transmembrane domains, and intracellular death domains.
  • Mutant forms of TNFRSF10A may include death domain-deleted or non-functional variants for specific studies.
• Promoter:
  • Constitutive Promoters: (e.g., CMV, EF1α) for robust expression.
  • Inducible Promoters: (e.g., tetracycline-inducible systems) for controlled expression.
  • Tissue-Specific Promoters: For targeting expression in specific cell types, such as immune cells or tumor cells.
• Tagging Sequences:
  • Fusion tags (e.g., FLAG, GFP, or His6) for protein tracking, detection, and purification.
• Selectable Markers:
  • Antibiotic resistance genes (e.g., ampicillin, kanamycin) for plasmid selection in bacterial or mammalian cells.

Applications of TNFRSF10A Plasmids

• Apoptosis Research:
  • TNFRSF10A plasmids are used to study TRAIL-induced apoptosis and the molecular mechanisms of DISC assembly and caspase activation.
• Cancer Research:
  • Investigate the role of TNFRSF10A in tumor cell sensitivity or resistance to TRAIL-mediated apoptosis.
  • Test strategies to enhance TNFRSF10A-mediated apoptosis in cancer cells using combination therapies or engineered TRAIL ligands.
• Signal Transduction Studies:
  • Analyze TNFRSF10A’s role in activating downstream signaling pathways, including caspase-dependent and non-apoptotic pathways like NF-κB.
• Therapeutic Screening:
  • Use TNFRSF10A plasmids to screen small molecules, antibodies, or TRAIL variants that enhance or inhibit TRAIL-R1 signaling.

TNFRSF10A plasmids are essential tools for exploring TRAIL-mediated apoptosis, immune regulation, and therapeutic strategies targeting the TRAIL signaling pathway. They provide insights into the molecular mechanisms of TNFRSF10A function and its role in cancer biology, immune modulation, and potential gene therapy applications.