Overview of TNF Plasmids

TNF Plasmids are genetic constructs designed to express the Tumor Necrosis Factor (TNF) gene in various cell types. TNF is a cytokine involved in systemic inflammation and is a key regulator of immune cells. It plays a significant role in the inflammatory response and is associated with various diseases, including cancer, autoimmune diseases, and infectious diseases.

Key Features

  • TNF Gene: The plasmid contains the gene sequence encoding the TNF protein. This sequence can be optimized for expression in different host cells.
  • Promoters: Strong promoters (e.g., CMV, EF1α) are included to drive high-level expression of the TNF gene.
  • Selectable Markers: Antibiotic resistance genes (e.g., ampicillin, neomycin) enable the selection of successfully transformed cells.
  • Multiple Cloning Sites (MCS): Allow the insertion of the TNF gene and other sequences of interest into the plasmid vector.
  • Fusion Tags: Some plasmids include tags (e.g., GFP, His-tag) for easy purification and visualization of TNF.

Applications

  • Inflammation Research: Study the role of TNF in the inflammatory response and its effects on various cell types.
  • Disease Models: Investigate the involvement of TNF in diseases such as cancer, rheumatoid arthritis, and inflammatory bowel disease by expressing TNF in relevant cellular and animal models.
  • Drug Development: Screen and evaluate potential therapeutic agents that target TNF signaling pathways.
  • Immune Response Studies: Explore the role of TNF in immune cell activation, differentiation, and function.

Methodology

  • Cloning: Insert the TNF gene into the plasmid vector using molecular cloning techniques such as restriction enzyme digestion and ligation.
  • Transformation: Introduce the plasmid into bacterial cells for propagation and amplification.
  • Transfection: Deliver the plasmid into mammalian cells to express the TNF protein.
  • Analysis: Use techniques such as ELISA, Western blotting, immunocytochemistry, and flow cytometry to study TNF expression, localization, and biological effects.
AffiPLASMID® pCMV-TNF (human) -EGFP-Neo
CAT# AFG-GNC-13461
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pCMV-TNF
CAT# AFG-GNC-13460
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pCMV-Lamp2 (mouse) -Tnf (mouse) -FLAG
CAT# AFG-GNC-11581
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pCMV6-TNF-3*FLAG
CAT# AFG-GNC-8332
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pT7- His- TNF
CAT# AFG-NVF-112507
Size: 2 μg
214.25 214.25 USD
AffiPLASMID® pET28a-rTNF
CAT# AFG-NVF-096477
Size: 2 μg
283.60 283.6 USD
AffiPLASMID® pET26b- TNF
CAT# AFG-NVF-096279
Size: 2 μg
214.25 214.25 USD
AffiPLASMID® pEGFP- TNF Plasmid
CAT# AFG-NVF-093011
Size: 2 μg
182.90 182.9 USD
AffiPLASMID® pCMV- TNF
CAT# AFG-NVF-077841
Size: 2 μg
214.25 214.25 USD
AffiPLASMID® pBV220-TNF
CAT# AFG-NVF-075679
Size: 2 μg
276.00 276.0 USD
AffiPLASMID® pBV220- TNF
CAT# AFG-NVF-075663
Size: 2 μg
214.25 214.25 USD
AffiPLASMID® Chicken TNF-α Expression Plasmid
CAT# AFG-GNS-2274
Size: 10 µg
218.05 218.05 USD