Overview of AICD Plasmids

AICD Plasmids are genetic constructs designed to express the Amyloid Precursor Protein Intracellular Domain (AICD) in various cell types. AICD is a fragment of the amyloid precursor protein (APP) that is generated through proteolytic processing and is implicated in cellular signaling, gene expression regulation, and Alzheimer's disease pathology.

Key Features

  • AICD Gene: The plasmid contains the gene sequence encoding the AICD fragment. This sequence can be optimized for enhanced expression in different host cells.
  • Promoters: Strong promoters (e.g., CMV, EF1α) are included to drive high-level expression of the AICD gene.
  • Selectable Markers: Antibiotic resistance genes (e.g., ampicillin, kanamycin) enable the selection of successfully transformed cells.
  • Multiple Cloning Sites (MCS): Allow the insertion of the AICD gene and other sequences of interest into the plasmid vector.
  • Fusion Tags: Some plasmids include tags (e.g., GFP, His-tag) for easy purification and visualization of AICD.

Applications

  • Alzheimer's Disease Research: Study the role of AICD in Alzheimer's disease pathology, including its effects on gene expression and cellular signaling pathways.
  • Gene Regulation Studies: Investigate how AICD influences the expression of various genes and its involvement in transcriptional regulation.
  • Cell Signaling: Explore the signaling pathways modulated by AICD and its interactions with other cellular proteins.
  • Neurobiology: Assess the broader implications of AICD in neuronal function and health.

Methodology

  • Cloning: Insert the AICD gene into the plasmid vector using molecular cloning techniques such as restriction enzyme digestion and ligation.
  • Transformation: Introduce the plasmid into bacterial cells for propagation and amplification.
  • Transfection: Deliver the plasmid into mammalian cells to express the AICD protein.
  • Analysis: Use techniques such as Western blotting, immunocytochemistry, qPCR, and reporter assays to study AICD expression, localization, and function.
AffiPLASMID® pCMV-Aicda (mouse) -3×FLAG-Neo
CAT# AFG-GNC-9029
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pCMV6-AICDA-3*FLAG
CAT# AFG-GNC-4072
Size: 1 Unit
For Price Contact info@affigen.com 0.0 USD
AffiPLASMID® pOTB7-AICDA
CAT# AFG-NVF-107680
Size: 2 μg
276.00 276.0 USD
AffiPLASMID® pECMV-Aicda-m-FLAG Plasmid
CAT# AFG-NVF-091738
Size: 2 μg
215.20 215.20000000000002 USD